Formulation and evaluation of herbal ointment using leaves of Thuja orientalis l. Leaf extract

The study of diseases and their treatment are important part of our ancient time worldwide. The knowledge of medicinal plants must have been accumulated in the course of many centuries. Herbal plants are huge sources of neutraceuticals, prevent the different disease or maintain healthy life. Plant produces primary metabolites for their basic survival and secondary metabolites for their ecological, taxonomical and biochemical differentiation and diversity. Herbal medicine prepare different part of plant are used. Herbal drug is design as the alternative formulation for the external use in the form of ointment. For the clinical use the herbal ointment apply externally on human body. The main aims of this research are preparation of herbal ointment from the Thuja orientalis L.Plants used as antibacterial activity. Under this research work, it is design as herbal ointment with the help of Ethanolic extract of Thuja orientalis L. and determines the anti bacterial capacity on it. In vitro study the antibacterial activity of the Ethanolic extracts of dried leaves of Thuja orientalis L. are determine by using the Agar cup plate method versus different bacteria such as Staphylococcus aureus, Bacillus subtilis, Escherichia coli and Pseudomonas aeruginosa etc. By blending the Ethanolic extract of Thuja orientalis L.(10 % w/w) into aqueous cream we formulated herbal ointment. The emulsifying agent and simple ointment bases are added in above herbal ointment for increase the antibacterial effectiveness during in vitro evaluation. Thuja orientalis L. (Family Cupressaceae) are broadly used in traditional system of medicine throughout different part of India, China, Russia, Korea, Japan, India, Florida and Iran. It is used in the treatment of diarrhea, cough, cold, bronchitis, and upper part repertory infection. The current study deals with the determination of formulation and evaluation of herbal ointment using Thuja orientalis leave extract.