Effects of culture medium composition on shoot multiplication and biomass production by in vitro culture of Dendrobium officinale (Kimura et Migo)

Dendrobium officinale (Kimura et Migo) is one of the most valuable and rare medicinal orchids exhausted in nature. The production of artificial plant biomass in this orchid is really necessary to prevent this risk. This study aimed to evaluate the effect of growth regulators (BAP, NAA, and GA3) on in vitro biomass production of Dendrobium officinale. Experiments were arranged in a completely randomized design (CRD) with three replicates. The results showed that MS medium supplemented with sucrose 30 gL-1, agar 6 gL-1, BAP gL-1, and NAA 0,5 gL-1 was the most suitable medium for in vitro biomass production. In vitro biomass of orchids cultured on the medium contained chemical components commonly found in wild dendrobium such as: Ca, Mg, protein, polysaccharides, and alkaloids. The success of this study is the basis for in vitro biomass production in order to supply materials for the medicine and food industries.